Precipitation Technique

                          Immuno chemical technique-  Precipitation                                        Made by- Vanie Singh                                        Department of Botany                                       Himachal   Pradesh university ·          Antigen reacts with antibodies to form the antigen-antibody complex. ·          Depending upon the size of nature of the complex and the nature of the antigen, two types of reactions are known 1.         Agglutination 2.         Precipitation ·          When a soluble antigen having at least 2 or more antibody binding sites react with the corresponding antibody in suitable concentration, it leads to the formation of large antigen-antibody complex which is visible to the naked eye as a precipitation reaction. Such reactions are called precipitation reactions. ·          On the other hand when the antigen is of particulate nature, its reaction with the antibody leads to visible clumping called agglutination.